Faculty

Brant Burkhardt

Associate Professor, MBS Graduate Director

CONTACT

Office: BSF 206
Phone: 813/974-5968
Lab: BSF 238, 240
Email

EDUCATION

  • B.S., College of New Jersey, Biology, 1993
  • M.S., Clemson University, Microbiology, 1996
  • Ph.D., University of Florida, Biomedical Sciences, 2001

PROFESSIONAL EXPERIENCE

Adjunct Professor, Burlington County College, 2010

Senior Research Associate, Children's Hospital of Philadelphia, 2006-2011

Post-Doc, University of Pennsylvania, 2003-2006

RESEARCH

Diabetes mellitus is a metabolic disease consisting of hyperglycemia caused by the failure of glucose regulation from either lack of insulin production (type 1 diabetes, T1D) or insulin sensitivity (type 2 diabetes, T2D). Specifically, T1D is an autoimmune disease culminating in the loss of pancreatic β-cells and insulin production. An estimated 1.25 million Americans have been diagnosed with T1D with about 18.5K youths being newly diagnosed every year. Recent studies have indicated that the incidence and prevalence of T1D are increasing globally.

Despite this unprecedented increase in T1D, interventional therapeutic options are almost nonexistent. There is currently only one very recently approved therapeutic, teplizumab, shown to inhibit T1D progression. However, the only available treatment following clinical diagnosis is lifelong exogenous insulin. Therefore, novel effective and affordable therapies are needed to preserve existing pancreatic β-cells from autoimmunity and the hyperinflammatory milieu to inhibit or ultimately prevent T1D. Our laboratory is focused on the discovery of novel therapeutics to inhibit or delay the progression of T1D. Evidence from our laboratory and others have indicated that bioactive ingredients within the affordable natural product of Cornus officinalis (CO) may inhibit T1D onset via stimulation of antioxidant pathways promoting pancreatic β-cell survival and decreased insulitis.

Cornus officinalis is a deciduous shrub or tree that is widely known for its fruit that contains a wide variety of medicinal value that has been used in Traditional Chinese Medicine (TCM) for over 2,000 years for a multitude of conditions. Our laboratory has published numerous papers revealing the therapeutic potential of C. officinalis in the early treatment of T1D by identifying the beneficial and protective effects via in-vitro, in-vivo and proteomic approaches.

Recent laboratory papers

Fletcher, J. D., Olsson, G. E., Zhang, Y. C., & Burkhardt, B. R. (2024). Oral gavage delivery of Cornus officinalis extract delays type 1 diabetes onset and hyperglycemia in non-obese diabetic (NOD) mice. FEBS Open Bio, 14(3), 434-443

Sharp-Tawfik, A.*, Fletcher, J. D.*, Guergues, J., Marelia-Bennett, C., Wolf, T. J., Coiner, A. M., Zhang , Y.C., Stevens S.M. & Burkhardt, B. R. (2022). Proteomic examination of Cornus officinalis stimulated 1.1B4 human pancreatic cells reveals activation of autophagy and Keap1/Nrf2 pathway. Mol Cell Endocrinol, 557, 111773. (* Co-First Author)

Sarkar, A., M. N. Kuehl, A. C. Alman, and B. R. Burkhardt. (2021). Linking the Oral Microbiome and Salivary Cytokine Abundance to Circadian Oscillations. Sci Rep 11, no. 1 : 2658.

Sharp-Tawfik, A. E., & Burkhardt, B. R. (2019). Chemical profile dataset of Cornus officinalis from multiple sources using HPLC/MS. Data Brief, 25, 104401.

Sharp-Tawfik, A. E., Coiner, A. M., MarElia, C. B., Kazantzis, M., Zhang, C., & Burkhardt, B. R. (2019). Compositional analysis and biological characterization of Cornus officinalis on human 1.1B4 pancreatic beta cells. Mol Cell Endocrinol, 494, 110491.

We have direct collaborations with both industry (Dr. John Chen, Evergreen Herbs) and medical professionals (Dr. Clare Zhang, Practice of Oriental Medicine) in the field of ethnopharmacology to optimize our approach and enhance our research.

Our laboratory is focused on identifying novel ethnopharmacological approaches along with their mechanism of action for the early interventional treatment of T1D.

usf collaborations

In addition to the major research focus on T1D of our laboratory, we also have active NIH funded collaborative research projects providing with Dr. Stan Stevens from Molecular Biosciences and Dr. Amy Alman from the College of Public Health. Dr. Stan Stevens utilizes proteomics to elucidate complex biological mechanisms underlying progression of human disease such as alcohol induced hepatic steatosis. Dr. Amy Alman investigates the biological relationships between inflammation, periodontal disease, diabetes, and cardiovascular disease.

GRADUATE STUDENTS

Justin Fletcher

underGRADUATE STUDENTS

Anna Wade, Sama Nayakanti

graduate student alumni

Name Degree Year Current Position
Grace Dougan MD- Fellowship 2013 Pediatric Endocrine Associates
Shari Moak MS-Bio 2013  
Whitney Ratliff Ph.D. 2015 NIH NCCIH Program Officer
Melanie Kuehl Ph.D. 2015 Scientist- Lonza
Mark Athanason Ph.D. 2016 Senior Scientist Merck
Katie MarElia-Bennett Ph.D. 2018 HLA Director-MUSC
Arielle Tawfik Ph.D. 2020 IVD Regulatory Affairs Scientist at Almac Group